Isolation of Listeria monocytogenes from Meat and Dairy Products
Authors
Abstract:
Introduction: This study was intended to determine the presence and distribution of Listeria monocytogenes in various meat and dairy products from Qazvin Province by culture followed by biochemical and morphological assays. The identity of the isolates was further obtained by amplification of prfA gene in bacteria isolates. This gene is a transcriptional activator of virulence gene expression within the pathogenic L. monocytogenes. Method: In a cross-sectional design, a total of 182 different food samples were collected from different areas in Qazvin, Iran. Bacterial isolates were obtained by the cold enrichment method. DNA extraction from the pelleted cells was conducted and then prfA gene was amplified by conventional PCR. Results: As many as 37 (20.3%) food samples were positive for Listeria spp. including 21 (56.8%) L. monocytogenes, 7 (18.9%) Listeria innocua, 4 (10.8%) Listeria welshimri, 3 (8.1%), Listeria seligeri, and 2 (5.4%) Listeria grayi. None of the isolated specimen was Listeria ivanovii. The PrfA gene was amplified in all L. monocytogenes specimen. Moreover, PCR assay had high sensitivity and specificity for bacterial identification. Conclusion: To sum up, presence of L. monocytogenes in food samples was confirmed in this region, it was more frequent in milk specimen. In addition to common culture techniques, PCR assay showed higher sensitivity and specificity for L. monocytogenes detection in contaminated foods.
similar resources
isolation of listeria monocytogenes from meat and dairy products
introduction: this study was intended to determine the presence and distribution of listeria monocytogenes in various meat and dairy products from qazvin province by culture followed by biochemical and morphological assays. the identity of the isolates was further obtained by amplification of prfa gene in bacteria isolates. this gene is a transcriptional activator of virulence gene expression w...
full textphenotypic and genotypic characteristics of listeria monocytogenes isolated from dairy and meat products
conclusions mlva analysis showed that samples obtained from different sources could have similar genetic profile. as a result, administration of penicillin in patients with listeriosis (especially pregnant women) and antibiotic susceptibility test are recommended. the fast and accurate methods such as mlva for tracking of pollution sources of l. monocytogenes are recommended during outbreaks. r...
full textAntimicrobial resistance of Listeria monocytogenes and Listeria innocua from meat products and meat-processing environment.
A total of 336 Listeria isolates from ready-to-eat (RTE) meat products and meat-processing environments, consisting of 206 Listeria monocytogenes, and 130 Listeria innocua isolates, were characterized by disc diffusion assay and minimum inhibitory concentration (MIC) values for antimicrobial susceptibility against twenty antimicrobials. Resistance to one or two antimicrobials was observed in 71...
full textPrevalence and Antibiotic Susceptibility of Listeria Monocytogenes Isolated from Retail Ready-to-Eat Meat Products in Gorgan, Iran
Background and Objectives: Listeria monocytogenes are known as an emerged foodborne pathogen and considered as a severe health risk. In the present study, prevalence and antibiotic resistance of L. monocytogenes isolated from ready-to-eat meat products in Iran were assessed. Materials and Methods: A total of 200 ready-to-eat meat products, including chicken meat (wing, breast, and leg), lamb a...
full textIsolation and Identification of Listeria monocytogenes from Retail Broiler Chicken Ready to Eat Meat Products in Sudan
Listeria species are widely distributed in environment and L. monocytogenes are the causal agent of Listeriosis, the disease that can be serious and fatal to human and animals. The objectives of this study were to detect, isolate and identify Listeria monocytogenes from retail broiler chicken ready to eat meat products in restaurants-Khartoum state, Sudan. A total of 250 retail broiler chicken ...
full textMy Resources
Journal title
volume 2 issue 4
pages 159- 162
publication date 2014-10
By following a journal you will be notified via email when a new issue of this journal is published.
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023